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For each qPCR reaction, 25 μl of master mix was prepared by adding 12.5 μl of the 2 × Universal SYBR (ThermoFisher) 5.5 μl of RNAse-free water, 1 μl ACX primer (0.05 μg /ul), 1 μl TS primer (0.1 μg /μl) (Table 1), and 4 μl of the protein sample

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In alcoholic liver disease, we see NFκB triggering monocytes and Kupffer cells in the hepatic sinusoids to release IL-6, IL-8, and TNF