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Amounts of vitamin B12 that are recommended by the Food and Nutrition Board, National Academy of Science-National Research Council for pregnant women (4 mcg daily) should be consumed during pregnancy

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To isolate chondrocytes, fresh articular cartilage tissues were rinsed with basal medium (BM), consisted of high glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Thermo Fisher Scientific, Waltham, MA, United States), with 2 × Antibiotics-Antimycotics (anti-anti, Life Technologies, Carlsbad, CA, United States) and then cut into ∼1 mm 3 pieces