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Strategies to improve the effectiveness of antioxidants are focused on chemical modifications of antioxidants (e.g., attachment of masking pegylated (PEG)-groups), coupling of antioxidants to affinity carriers (e.g., antibodies against cellular adhesion molecules) and drug-delivery systems such as micelles and liposomes [4, 13–15, 17]

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The samples were centrifuged by 5 min, at 4 °C, using a speed 12 000 rpm